Moreover, digoxin was shown to induce changes in intracellular membrane traffic in neuronal cells, whereas ouabain does not possess this ability and even antagonized digoxin effect. 10 Scopus citations. were ofthe order of 10-6 for ouabain and somewhat higher for digoxin. In a second study, BP increased in ouabain (15 μg/kg/day, n=23), but not digoxin (30 μg/kg/day, n=12), or vehicle-infused (n=16) rats. Digoxin and Fab fragments had no effects on either function. As digoxin and ouabain increase the synthesis of cholesterol, one might expect that these cells ‘fill up’ with cholesterol. Both ouabain and digoxin stimulated expression of the alpha 3-isoform, whereas alpha 2 was unchanged in those two groups. Ouabain and digoxin activate the cellular proteasome, instigating ER degradation, which causes the inhibition of 17 -estradiol signaling, induces the cell cycle blockade in the G2 phase, and triggers apoptosis. rat: ouabain: 24 % digoxin: 75 % digitoxin: 86 %, guinea pig: ouabain: 48 %, digoxin: 20 % (! The improvement results from the use of ouabain rather than digoxin as the means for separating the bound and free labeled components. Research output: Contribution to journal › Article. Digoxin: The excretion of Ouabain can be decreased when combined with Digoxin. Digoxin and ouabain reduced the influx and had no appreciable effect on the efflux ofpotassium, so that the auricles lost potassium. Digoxin: A cardiac glycoside used in the treatment of mild to moderate heart failure and for ventricular response rate control in chronic atrial fibrillation. Ouabain and digoxin activate the cellular proteasome, instigating ERα degradation, which causes the inhibition of 17β-estradiol signaling, induces the cell cycle blockade in the G2 phase, and triggers apoptosis. Overview; Fingerprint; Abstract . Compared to digoxin, ouabain is more hydrophilic and cannot easily cross the Brain–Blood Barrier (BBB) (Sugimoto et al., 2011). It lowers BP in ouabain- and adducin-dependent hypertension in rats and is a promising new class of antihypertensive medication in humans. The threshold concentrations which produced effects within 20 min. Indeed, H9c2 cells treated with digoxin and ouabain had low levels of intracellular cholesterol and showed a time-dependent cholesterol accumulation in the culture medium. There are marked differences between the effects of digoxin and ouabain. To examine acute ouabain–digoxin interactions, we tested these and related CTSs on myogenic tone (MT) in pressurized rat mesenteric small arteries and glutamate‐evoked Ca 2+ transients in primary cultured rat hippocampal neurones. Digoxin is known to be secreted by renal tubular cells, but the mechanisms are still not fully understood. Irregularities or failure of contraction occurred when the tissue potassium was reduced by about 15%. Ouabain has become the standard tool to investigate the mode of action of cardiotonic steroids, and results with ouabain are regarded as generally valid for all cardiac glycosides. Digoxin, ouabain, zaragozic acid and the BCA (bicinchoninic acid) kit for protein measurement were from Sigma. !e half-maximal inhibitory concentration (IC50) of ouabain and digoxin were determined at a nanomolar concentration. In the presence of calcium, the glycosides only affected potassium uptake and histamine release slightly. In comparison with chloroquine, ouabain and digoxin were much more e"ective. (A) Structural representation of the CTSs digoxin, bufalin, and ouabain. Comparative Cardiotoxicity of Low Doses of Digoxin, Ouabain, and Oleandrin. Abstract—Digoxin prevents ouabain-induced hypertension in rats. Remarkably, these e ects are independent of the inhibition of the Na/K pump. Remarkably, these effects are independent of the inhibition of the Na/K pump. The exact mechanism of action of these drugs has remained an enigma. Dihydrostreptomycin: The risk or severity of adverse effects can be increased when Dihydrostreptomycin is combined with Ouabain. 1. Cardiac glycosides are promising anticancer drugs. bufalin, digoxin, and ouabain (5) is depicted in blue, green, and gray cartoons, respectively, and the bufalin, digoxin, and ouabain molecules are represented by magenta, orange, and dark gray sticks, respectively. activity of ouabain and digoxin against SARS-CoV-2 infection [8]. We have recently shown that the cardiac glycosides digitoxin, digoxin and ouabain induce selective killing of lung cancer cells, and that the cytotoxicity of digitoxin against these cells occurs at concentrations below those observed in the plasma of cardiac patients treated with this drug (Oncogene, 2013. doi: 10.1038/onc.2013.229). Ouabain and digoxin enhanced the histamine release while digitoxigenin either had no effect or was slightly inhibitory. Such occurrence, however, has never been described in patients treated with digoxin. Our results suggest that weight loss during opiate withdrawal is mediated … Dihydrotachysterol: The risk or severity of ventricular arrhythmias and Cardiac Arrhythmia can be increased when Dihydrotachysterol is combined with Ouabain. Cardiovasc Toxicol. Therefore, the pharmacological action of ouabain is more peripheral, which explains why it has no effect on locomotion in mice. A heterogeneous immunoassay for digoxin having improved sensitivity and precision is disclosed. Nevertheless, prolonged ouabain, but not digoxin, administration induces hypertension; moreover, digoxin antagonizes the hypertensinogenic effect of ouabain. Digoxin and digitoxin are widely used in the treatment of heart diseases. Digoxin for 24 h and 48 h of incubation in MDA-MB-231 cells proved to be only slightly more potent than ouabain, with IC50 values of 122 ± 2 and 70 ± 2 nM, respectively, compared to 150 ± 2 and 90 ± 2 nM for ouabain. Digoxin immune Fab protects endothelial cells from ouabain‐induced barrier injury. Digoxin and ouabain reduced the influx and had no appreciable effect on the efflux of potassium, so that the auricles lost potassium. They may also have additional effects, such as on metabolism of steroid hormones, although until now no evidence has been provided about the effects of these cardioactive glycosides on the synthesis of cholesterol. In an in vitro assay for the inhibitory effects on Na ϩ ,K ϩ -ATPase activity, 20 ng of ouabain caused 29% inhibition, but 20 ng of ouabain plus 13 or 53 ng of digoxin caused only 16% or 4% inhibition, respectively. In the presence of lithium or lanthanum the enhancement of the histamine release was counteracted. Thethreshold concentrations whichproduced effects within 20 min. were of the order of 10 −6 for ouabain and somewhat higher for digoxin. Am J Reprod Immunol 2012; 67: 66–72. Robert William Caldwell, C. B. Nash. Plasma ouabain, BP, and all evoked responses were normal one week following interruption of the ouabain infusion. This work was aimed at determining the cardioprotective effect of digitalis glycosides in rat heart, and to relate it with Na+, K+-ATPase inhibition and ERK1/2 activation. Milrinone does not affect locomotion in mice either. Comparison of the effects of aminosugar cardiac glycosides wth ouabain and digoxin on Na+, K+ adenosine triphosphatase and cardiac contractile force. Problem Endogenous digitalis‐like factors (EDLF) inhibit sodium pump Na + /K + ATPase activity, and maternal EDLF levels are elevated in preeclampsia (PE). In this study, we examined renal tubular cell handling of digoxin and ouabain using LLC-PK1 cells, a model of proximal renal tubular cells. alpha 1-isoform expression decreased in the ouabain group and was unchanged in the digoxin group. Digoxin, which is synthesized in adrenal glands, seems to counteract the hypertensinogenic action of ouabain in rats, as do antibodies against ouabain, for example, (Digibind) and rostafuroxin (PST 2238), a selective ouabain antagonist. Deslanoside: For the treatment and management of Congestive cardiac insufficiency, arrhythmias and heart failure. 2. Digoxin and ouabain are steroid drugs that inhibit the Na+/K+-ATPase, and are widely used in the treatment of heart diseases. Plasma and kidney levels of ouabain immunoreactivity were increased 4-8 fold in ouabain infused rats while blood pressure and plasma levels of ouabain returned to normal one week following discontinuation of the steroid infusion. Virus titers of single dose treatment of ouabain and digoxin showed a >99% inhibition of SARS-CoV-2 replication. ), digitoxin: 59 % Marzo et al. Activation of c-SRC underlies the differential effects of ouabain and digoxin on Ca2+ signaling in arterial smooth muscle cells Poisoning the hearts of dogs and of dog heart-lung preparations with ouabain or digoxin to the extent of producing ventricular tachycardia or ventricular fibrillation caused a severe depletion of the phosphocreatine stores of the tissue. Comparative Cardiotoxicity of Low Doses of Digoxin, Ouabain, and Oleandrin. 2020 Jun 01;: Authors: Botelho AFM, Miranda ALS, Freitas TG, Milani PF, Barreto T, Cruz JS, Melo MM Abstract The aim of this study was to evaluate the comparative effects of CGs on heart physiology. The cells were used in suspension for binding experiments and in monolayers on permeable filters for transport studies. The K +and Mg2 ions are represented by purple and yellow spheres, respectively. When not otherwise specified, all of the other reagents were purchased from Sigma. DLSTRISUTION OF OUABAIN AND DIGOXIN IN THE RAT AND GUINEA PIG S. Dutta and B. H. Marks Department of Pharmacology, Ohio State University College of Medicine, Columbus, Ohio (Received 14 February 1966; is final form 21 1larch 1966) THE pattern of distribution of various digitaloids has been studied by a number of investigators ( 1, 2, 3) . The ATP levels were slightly lowered or remained unchanged. December 2020; Cardiovascular Toxicology 20(1) DOI: 10.1007/s12012-020-09579-1. Acetyldigitoxin: Used for fast digitalization in congestive heart failure. Only ouabain in small doses stimulates the sodium pump; digoxin does not show this effect. Nevertheless, prolonged ouabain, but the mechanisms are still not fully.. Influx and had no effect or was slightly inhibitory were determined at a nanomolar concentration (! Sars-Cov-2 infection [ 8 ] mechanisms are still not fully understood an.! Ventricular arrhythmias and heart failure either function the order of 10 −6 for ouabain and digoxin were more. The alpha 3-isoform, whereas alpha 2 was unchanged in those two groups digoxin, administration induces hypertension ;,. Reprod Immunol 2012 ; 67: 66–72, one might expect that these cells ‘ fill up ’ with.... Were determined at a nanomolar concentration the other reagents were purchased from Sigma the Na/K pump has remained enigma... 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